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Cancer Cell International
·Localization of CD26/DPPIV in nucleus and its nuclear translocation enhanced by anti-CD26 monoclonal antibody with anti-tumor effect
·Eurycomanone induce apoptosis in HepG2 cells via up regulation of p53
·Bioluminescence imaging reveals inhibition of tumor cell proliferation by Alzheimer's amyloid beta protein
·Acetoacetate reduces growth and ATP concentration in cancer cell lines which over-express uncoupling protein 2
·Nucleic acid distribution pattern as a possible biomarker for metabolic activities of neoplastic cells: a digitally-aided fluorescence microscopy study on normal and neoplastic lymphocytes of acute and chronic canine lymphocytic leukemia
·Gene expression profiling predicts a three-gene expression signature of endometrial adenocarcinoma in a rat model
·Down-regulation of SFRP1 in a mammary epithelial cell line promotes the development of a CD44high/CD24low population which is invasive and resistant to anoikis
·Decytabine enhances cytotoxicity induced by oxaliplatin and 5-fluorouracil in the colorectal cancer cell line Colo-205.
·Pegylated derivatives of recombinant human arginase (rhArg1) for sustained in vivo activity in cancer therapy: preparation, characterization and analysis of their pharmacodynamics in vivo and in vitro and action upon hepatocellular carcinoma cell (HCC)
·Mammaglobin as a potential molecular target for breast cancer drug delivery

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Immunohistochemistry - In Situ Hybridization



Immunohistochemistry


Immunohistochemistry is a technique for identifying cellular or tissue constituents (antigens)
by means of antigen-antibody interactions, the site of antibody binding being identified
either by direct labelling of the antibody, or by use of a secondary labelling method.

In Situ Hybridization techniques allow the demonstration of specific nucleic
acid sequences (genes) in their cellular environment.

Above: Human Papillomavirus DNA demonstrated by In Situ Hybridisation (pink)
in epithelial cells identified by indirect immunofluorescence using antibody against cytokeratin (green)



FISH, Fluorescent In Situ Hybridization In Situ Hybridization, Chromosome1 In Situ Hybridization, HHV8 FISH, Fluorescent In Situ Hybridization, HPV


Immunohistochemistry Protocols Immunohistochemistry Protocols (Web Links)

In Situ Hybridization Protocols (Web Links)

Discuss Immunohistochemistry & In Situ Hybridization

Exploring the functional neuroanatomy of neuronal nicotinic acetylcholine receptors - Immunolabelling Methods

Immunohistochemistry Positive Control Tissue

Immunohistochemistry Image Gallery




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Modern Immunohistochemistry
ImmunohistochemistryModern Immunohistochemistry (Cambridge Illustrated Surgical Pathology)

Diagnostic pathology is an inherently flawed science due to the many possible interpretations of various tissues. However, the application of immunohistochemical stains as a diagnostic tool has been widely used since the 1990s as an extremely effective ancillary technique that removes much subjectivity from the practice. In fact, immunohistochemistry has supplanted simple morphologic evaluation as the definitive diagnostic method for a wide array of tumor types.
This book offers a new and modern atlas-based resource for this science. Every anatomic region is covered in detail, and major diseases contain side-by-side examples of other ancillary staining techniques for comparison. The text is geared toward both the resident and practitioner of anatomic pathology and is supplemented with histograms, algorithms, and guides to the application and interpretation of uncommon antigens and immunostains. Not only is the book illustrated with more than 600 high-quality photomicrographs, but a companion CD-ROM of all images in downloadable format is included.

Published on: 2009-04-27

Immunohistochemistry Book Store

Posted by admin on Friday, June 19 @ 21:13:45 CEST (62 reads)
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Immunohistochemistry - NeuroMabs
Immunohistochemistry The mission of the NeuroMab facility is to provide a unique neuroscience-based approach to generating mouse monoclonal antibodies optimized for use in mammalian brain (NeuroMabs). NeuroMabs are generated from mice immunized with synthetic and recombinant immunogens corresponding to components of the neuronal proteome as predicted from genomic and other large-scale cloning efforts. Comprehensive biochemical and immunohistochemical analyses of human, primate and non-primate mammalian brain are incorporated into the initial NeuroMab screening procedure. This yields a subset of mAbs that are optimized for use in the brain for immunocytochemical-based imaging studies of protein localization in adult, developing and pathological brain samples, for biochemical analyses of subunit composition and post-translational modifications of native brain proteins, and for proteomic analyses of native brain protein networks.

The initial goal of the facility for the next 5 years is to generate a library of novel NeuroMabs against 250-500 neuronal proteins, initially focusing on membrane proteins (receptors/channels/transporters), synaptic proteins, other neuronal signaling molecules, and proteins with established links to disease states. These NeuroMabs will then be produced on a large scale and made available to the neuroscience research community on an inexpensive basis as tissue culture supernatant or purified immunoglobulin by Antibodies Inc.

NeuroMab website at www.NeuroMab.org

Posted by admin on Monday, March 09 @ 08:00:00 CET (345 reads)
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Video Protocols
ImmunohistochemistryThe Journal of Visualized Experiments (JoVE)

The Journal of Visualized Experiments (JoVE) has announced that its online video protocols will be indexed in the popular US National Library of Medicine repositories MEDLINE and PubMed.
Founder and chief executive Moshe Pritsker views the MEDLINE–PubMed listing as a sign that the scientific community has accepted video-based publications. "It was a very important decision for us, and for scientific publishing," he says.
Since JoVE was founded in 2006 with support from an angel investor, the journal has published more than 200 videos, most produced by professional videographers. It aims to improve the reproducibility of scientific results by using videos to clarify subtle experimental details. The journal was itself an experiment in video publishing and remains the only video-based scientific journal.


Immunocytochemistry: Human Neural Stem Cells
Immunocytochemistry is a very powerful and fairly straightforward method for determining the presence, subcellular localization, and relative abundance of an antigen of interest, most commonly a protein, in cultured cells. This protocol presents an easy-to-follow series of steps that will enable researchers to conserve primary and secondary antibodies while getting high quality, reproducible qualitative and quantitative data out of their staining. There are two aspects of this protocol that help to conserve the volume of antibody necessary for staining. For one, the cells are grown on small, circular coverslips that are placed in wells of a tissue culture plate. After fixation, the cells on coverslips can be removed from the wells of the plate. For antibody staining, the coverslip with cells is inverted onto a small drop of antibody solution on parafilm and is covered with a second piece of parafilm to prevent drying. Using this method, only ~25 μl of antibody solution is needed for each coverslip (or sample) to be stained. This protocol describes immunostaining of human neural stem/precursor cells (hNSPCs), but can be used for many other cell types.

More Protocols from JoVE

Posted by admin on Wednesday, March 04 @ 11:44:39 CET (865 reads)
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In Situ Hybridization
In Situ HybridizationLocked nucleic acid (LNA™)


This is a presentation of Exiqons miRCURY LNA™ microRNA Detection Probes for In Situ Hybridization.




Locked nucleic acid (LNA™) nucleosides are a class of nucleic acid analogues in which the ribose ring is “locked” by a methylene bridge connecting the 2’-O atom and the 4’-C atom. LNA™ nucleosides contain the six common nucleobases (T, C, G, A, U and mC) that appear in DNA and RNA and are able to form base pairs according to standard Watson-Crick base pairing rules. However, by “locking” the molecule with the methylene bridge the LNA™ is constrained in the ideal conformation for Watson-Crick binding. When incorporated into a DNA oligonucleotide, LNA™ therefore makes the pairing with a complementary nucleotide strand more rapid and increases the stability of the resulting duplex.

LNA™ Oligonucleotides
An LNA™-enhanced oligonucleotide is the ideal choice whenever short or very similar sequences need to be analyzed. The high affinity of an LNA™ oligonucleotide to its complementary sequence results in higher specificity and sensitivity than when using traditional DNA or RNA sequences. In many cases, LNA™-enhanced oligonucleotides can distinguish between sequences differing by a single nucleotide, which can be critical for the success of many experiments.


www.exiqon.com

Posted by admin on Friday, February 20 @ 08:55:25 CET (477 reads)
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Immunohistochemistry & In Situ Hybridization
ImmunohistochemistrySignal Amplifiers
UltraAmp™ Multi-Assay Signal Amplifiers


Genisphere’s UltraAmp Multi-Assay Signal Amplifier opens up endless possibilities for high sensitivity detection. UltraAmp reagents are available with multiple different detection labels and targeting moieties, and come in different sizes to meet your specific assay design. The sensitivity improvement obtained with this single tube reagent is up to 200 fold, depending on the size of dendrimer, application, and nature of the assay. Genisphere has tested the UltraAmp products in several different assays (protein arrays, ELISA, Bead Flow (Luminex), and Nucleic Acid Arrays), to show the versatility of the detection reagent, but ultimately the application is up to you.



Genisphere’s new universal UltraAmp Amplification reagents drop right into your existing assay and improve your sensitivity up to 200 fold! These reagents come with different labels, target moieties and sizes to accommodate limitless assays’ needs for more sensitivity. There are over 70 different UltraAmp varieties to choose from.

Read more at www.genisphere.com

Posted by admin on Saturday, February 14 @ 19:15:27 CET (520 reads)
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Applied Immunohistochemistry & Molecular Morphology

·New Website for Applied Immunohistochemistry Molecular Morphology Moves the Journal to the Forefront of Electronic Medical Publishing
·Merkel Cell Carcinoma: Lack of KIT Positivity and Implications for the Use of Imatinib Mesylate
·Apoptotic Mechanisms in Fulminant Hepatic Failure: Potential Therapeutic Target
·CEACAM1 Expression in Pancreatic Endocrine Tumors
·[beta]-catenin Expression is Altered in Dysplastic and Nondysplastic Aberrant Crypt Foci of Human Colon
·Primary and Secondary T-cell Lymphomas of the Breast: Clinico-pathologic Features of 11 Cases
·Expression of VEGF in Urinary Bladder Transitional Cell Carcinoma in an Iraqi Population Subjected to Depleted Uranium: An Immunohistochemical Study
·Collagen Type I may Influence the Expression of E-Cadherin and Beta-catenin in Carcinoma Ex-pleomorphic Adenoma
·Role of HERV-W Syncytin-1 in Placentation and Maintenance of Human Pregnancy
·Development of an Unsupervised Pixel-based Clustering Algorithm for Compartmentalization of Immunohistochemical Expression Using Automated QUantitative Analysis
·Malignant Histiocytoses/Disseminated Histiocytic Sarcoma With Hemophagocytic Syndrome in a Patient With Mediastinal Germ Cell Tumor
·Evidence for Transformation of Fibroadenoma of the Breast to Malignant Phyllodes Tumor
·Expression of Galectin-3, CD138, p16INK4a, and TTF-1 in Mucinous Bronchioloalveolar Adenocarcinoma After Hodgkin Lymphoma
·Immunohistochemical Marker for Na+ CP Type V[alpha] (C-20) and Heterozygous Nonsense SCN5A Mutation W822X in a Sudden Cardiac Death Induced by Mild Anaphylactic Reaction

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Applied Immunohistochemistry - Published Ahead-of-Print

·Are the Pure In Situ Breast Ductal Carcinomas and Those Associated With Invasive Carcinoma the Same?
·Utility of Fascin and JunB in Distinguishing Nodular Lymphocyte Predominant From Classical Lymphocyte-rich Hodgkin Lymphoma
·Determining Tissue of Origin for Metastatic Cancers: Meta-analysis and Literature Review of Immunohistochemistry Performance
·Effect of Cytotoxic Agents and Retinoic Acid on Myc-N Protein Expression in Neuroblastoma
·Large Cell Carcinoma of the Lung: An Endangered Species?
·Trefoil Peptides, E-cadherin, and [beta]-catenin Expression in Sporadic Fundic Gland Polyps: Further Evidence Toward the Benign Nature of These Lesions
·Cytokeratin Immunoexpression in Esophageal Squamous Cell Carcinoma of High-risk Population in Northeast India
·Significance of CD23 Expression in Diffuse Areas of Follicular Lymphoma
·Pancreatic Expression of DOG1: A Novel Gastrointestinal Stromal Tumor (GIST) Biomarker
·Computer-aided Detection of Prostate Cancer on Tissue Sections
·Cytokeratin-positive Cells in the Bone Marrow of Breast Cancer Patients and Noncancer Donors
·Estrogen and Progesterone Receptor Expression is not Always Specific for Mammary and Gynecologic Carcinomas: A Tissue Microarray and Pooled Literature Review Study
·FOXP1 and BCL2 Show Similar Immunoenzymatic Pattern in Bone Marrow Trephines of Chronic Lymphocytic Leukemia Patients
·Nondestructive Quality Control of HER2 Control Cell Line Sections: The use of Interferometry for Measuring Section Thickness and Implications for HER2 Interpretation on Breast Tissue
·Diagnostic Utility of Nestin Expression in Pediatric Tumors in the Region of the Kidney
·Survivin Expression Quantified by Image Pro-plus Compared With Visual Assessment
·Characterization of High-grade Ductal Carcinoma In Situ With and Without Regressive Changes: Diagnostic and Biologic Implications
·Potential Role of Tissue Microarrays for the Study of Biomarker Expression in Benign Breast Disease and Normal Breast Tissue
·Implementation of a Canadian External Quality Assurance Program for Breast Cancer Biomarkers: An Initiative of Canadian Quality Control in Immunohistochemistry (cIQc) and Canadian Association of Pathologists (CAP) National Standards Committee/Immunohistochemistry
·Attenuated Podoplanin Staining in Breast Myoepithelial Cells: A Potential Caveat in the Diagnosis of Lymphatic Invasion
·Immunohistochemical Evaluation of FLI-1 in Acute Lymphoblastic Lymphoma (ALL): A Potential Diagnostic Pitfall
·Malignant Solitary Fibrous Tumor: Report of 3 Cases With Unusual Features
·Instability of Induction Cooker (Electromagnetic Stove) Antigen Retrieval in Immunohistochemistry
·Utility of Flow Cytometry Immunophenotyping in Fine-needle Aspirate Cytologic Diagnosis of Non-Hodgkin Lymphoma: A Series of 252 Cases and Review of the Literature
·Translocations Involving MUM1 are Rare in Diffuse Large B-cell Lymphoma
·E-cadherin and [beta]-catenin Loss of Expression Related to Bone Metastasis in Prostate Cancer

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Applied Immunohistochemistry - Most Popular Articles

· Consensus Recommendations on Estrogen Receptor Testing in Breast Cancer By Immunohistochemistry
· Papillary and Follicular Neoplasms of the Thyroid Gland: Differential Immunohistochemical Staining With High-Molecular-Weight Keratin and Involucrin
· Automation of Manual Components and Image Quantification of Direct Dual Label Fluorescence In Situ Hybridization (FISH) for HER2 Gene Amplification: A Feasibility Study
· Comparison of Immunoexpression of 2 Antibodies for Estrogen Receptors (1D5 and 6F11) in Breast Carcinomas Using Different Antigen Retrieval and Detection Methods
· Characterization and Applications of a Newly Developed Rabbit Monoclonal Antibody to Cytokeratin 7 (CK7) for Immunohistochemistry
· Increasing the Efficiency of Paraffin Tissue Microarrays by Packing the Paraffin Tissue Core Biopsies in a Honeycomb Pattern
· Background Staining of Visualization Systems in Immunohistochemistry: Comparison of the Avidin-Biotin Complex System and the EnVision+ System
· Experimental Validation of Peptide Immunohistochemistry Controls
· CD5 Negative, Cyclin D1-positive Diffuse Large B-cell Lymphoma (DLBCL) Presenting as Ruptured Spleen
· Recommendations for Improved Standardization of Immunohistochemistry
· Systematic Review of HER2 Breast Cancer Testing
· Molecular Markers in Thyroid Fine-needle Aspiration Biopsy: A Prospective Study
· Microwave Antigen Retrieval Blocks Endogenous Peroxidase Activity in Immunohistochemistry
· Multiparameter Immunofluorescence on Paraffin-Embedded Tissue Sections
· Rab3B Immunoexpression in Human Pituitary Adenomas
· Snap-Frozen Brain Tissue Sections Stored With Desiccant at Ambient Laboratory Conditions Without Chemical Fixation are Resistant to Degradation for a Minimum of 6 Months
· Fixation and Epitope Retrieval in Diagnostic Immunohistochemistry: A Concise Review with Practical Considerations
· CDX2, Cytokeratins 7 and 20 Immunoreactivity in Rectal Adenocarcinoma
· Validation of a low Cost Computer-based Method for Quantification of Immunohistochemistry-stained Sections
· ALK-positive Diffuse Large B-cell Lymphoma With the t(2;17)(p23;q23)
· A Novel Multipurpose Monoclonal Antibody for Evaluating Human c-Met Expression in Preclinical and Clinical Settings
· Immunohistochemical Comparison of Chordoma With Chondrosarcoma, Myxopapillary Ependymoma, and Chordoid Meningioma
· Development of Standard Estrogen and Progesterone Receptor Immunohistochemical Assays for Selection of Patients for Antihormonal Therapy
· Labeling of Multiple Cell Markers and mRNA Using Automated Apparatus
· Determination of EGFR Status in Gliomas: Usefulness of Immunohistochemistry and Fluorescent In Situ Hybridization
· Effects of Fixative and Fixation Protocols on Assessment of Her-2/neu Oncogene Amplification Status by Fluorescence In Situ Hybridization
· One-step Complete Melting of Paraffin Tissue Microarrays Using Stabilization Bodies
· An Alternative and Reliable Real-time Quantitative PCR Method to Determine HER2/neu Amplification in Breast Cancer
· Immunohistochemistry of Tissue Prepared by a Molecular-Friendly Fixation and Processing System
· Epithelioid Sarcoma of the Vulva in a 17-year-old Pregnant Woman
· Sensitivity and Detection Efficiency of a Novel Two-Step Detection System (PowerVision) for Immunohistochemistry
· Immunoprofile of Adenocarcinomas of the Endometrium, Endocervix, and Ovary With Mucinous Differentiation
· Comparison of Quantification of Histochemical Staining By Hue-Saturation-Intensity (HSI) Transformation and Color-Deconvolution
· KIT (CD117): A Review on Expression in Normal and Neoplastic Tissues, and Mutations and Their Clinicopathologic Correlation
· Utility of Antiphosphorylated H2AX Antibody ([gamma]-H2AX) in Diagnosing Metastatic Renal Cell Carcinoma
· Detection of HPV-induced Cervical (Pre) Neoplastic Lesions: A Tissue Microarray (TMA) Study
· pH-dependent Antigen Unmasking in Paraformaldehyde-fixed Tissue Cryosections
· Use of Commercially Available Rabbit Monoclonal Antibodies for Immunofluorescence Double Staining
· HER2/Neu Detection by Immunohistochemistry: Optimization of In-house Protocols
· Diagnosis of Mycosis Fungoides: A Comparative Immunohistochemical Study of T-Cell Markers Using a Novel Anti-CD7 Antibody
· Effect of Storage on p63 Immunohistochemistry: A Time-course Study
· Development of New Rabbit Monoclonal Antibody to Progesterone Receptor (Clone SP2): No Heat Pretreatment but Effective for Paraffin Section Immunohistochemistry
· The Effects of Tissue Fixation Alternatives on DNA Content: A Study on Normal Colon Tissue
· Oncocytoma Can be Differentiated From its Renal Cell Carcinoma Mimics by a Panel of Markers: An Automated Tissue Microarray Study
· Analysis of Immunohistochemical Markers in Bone Marrow Sections to Evaluate for Myelodysplastic Syndromes and Acute Myeloid Leukemias
· Real-time PCR Analysis of RNA Extracted From Formalin-fixed and Paraffin-embeded Tissues: Effects of the Fixation on Outcome Reliability
· CDX2 Immunostaining as a Gastrointestinal Marker: Expression in Lung Carcinomas Is a Potential Pitfall
· Primary Cerebral Diffuse Large B-cell Lymphoma Relapsed Solely in the Skin With the Same Clonal Origin
· Albumin in Immunohistochemistry: Foe and Friend
· Pancreatic Expression of DOG1: A Novel Gastrointestinal Stromal Tumor (GIST) Biomarker

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The Journal of Pathology

·Tumour suppression by p53: the importance of apoptosis and cellular senescence
·Genome-wide transcriptomic profiling of microdissected human breast tissue reveals differential expression of KIT (c-Kit, CD117) and oestrogen receptor-[alpha] (ER[alpha]) in response to therapeutic radiation
·A potentially dynamic lysosomal role for the endogenous TRPML proteins
·Progranulin expression correlates with dense-core amyloid plaque burden in Alzheimer disease mouse models
·Down-regulated expression of SATB2 is associated with metastasis and poor prognosis in colorectal cancer
·The haematopoietic specific signal transducer Vav1 is aberrantly expressed in lung cancer and plays a role in tumourigenesis
·[beta]1 integrin/FAK/ERK signalling pathway is essential for human fetal islet cell differentiation and survival
·Dominant negative factors in health and disease
·Transcription factor Nfat1 deficiency causes osteoarthritis through dysfunction of adult articular chondrocytes
·Mononucleotide precedes dinucleotide repeat instability during colorectal tumour development in Lynch syndrome patients
·Development of oligomeric prion-protein aggregates in a mouse model of prion disease
·Reduced expression and novel splice variants of ING4 in human gastric adenocarcinoma
·Neuronal differentiation in basal cell carcinoma: possible relationship to Hedgehog pathway activation?
·Altered expression of MUTYH and an increase in 8-hydroxydeoxyguanosine are early events in ulcerative colitis-associated carcinogenesis
·MUTYH-associated polyposis carcinomas frequently lose HLA class I expression - a common event amongst DNA-repair-deficient colorectal cancers
·Increased RANKL expression is related to tumour migration and metastasis of renal cell carcinomas
·Morphological alterations to neurons of the amygdala and impaired fear conditioning in a transgenic mouse model of Alzheimer's disease
·Bone loss in survival motor neuron (Smn-/- SMN2) genetic mouse model of spinal muscular atrophy
·Oral rapamycin reduces tumour burden and vascularization in Lkb1+/- mice
·Bone morphogenetic protein 4 is induced in hepatocellular carcinoma by hypoxia and promotes tumour progression
·Genomic and phenotypic analysis reveals a key role for CCN1 (CYR61) in BAG3 - modulated adhesion and invasion
·PTEN genomic deletion is associated with p-Akt and AR signalling in poorer outcome, hormone refractory prostate cancer
·The histone deacetylase inhibitor LBH589 inhibits expression of mitotic genes causing G2/M arrest and cell death in head and neck squamous cell carcinoma cell lines
·Quantitative cell signalling analysis reveals down-regulation of MAPK pathway activation in colorectal cancer
·[Delta]Np63 isoforms differentially regulate gene expression in squamous cell carcinoma: identification of Cox-2 as a novel p63 target
·COX-2 is induced by HGF stimulation in Met-positive thyroid papillary carcinoma cells and is involved in tumour invasiveness
·Molecular characterization of post-transplant lymphoproliferative disorders of donor origin occurring in liver transplant recipients
·Obestatin in human neuroendocrine tissues and tumours: expression and effect on tumour growth
·Fibrillin-1 staining anomalies are associated with increased staining for TGF-[beta] and elastic fibre degradation; new clues to the pathogenesis of emphysema
·Nicotinic receptor-associated modulation of stimulatory and inhibitory neurotransmitters in NNK-induced adenocarcinoma of the lungs and pancreas
·FoxM1 is up-regulated in gastric cancer and its inhibition leads to cellular senescence, partially dependent on p27kip1

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Modern Pathology

·HLA-G polymorphisms in women with squamous intraepithelial lesions harboring human papillomavirus
·Chromosomal instability, telomere shortening, and inactivation of p21WAF1/CIP1 in dysplastic nodules of hepatitis B virus-associated multistep hepatocarcinogenesis
·Detection and characterization of EWSR1/ATF1 and EWSR1/CREB1 chimeric transcripts in clear cell sarcoma (melanoma of soft parts)
·A novel five-antibody immunohistochemical test for subclassification of lung carcinoma
·Pseudosarcomatous fibroblastic/myofibroblastic proliferation in perinephric adipose tissue adjacent to renal cell carcinoma: a lesion mimicking well-differentiated liposarcoma
·Poor prognostic significance of unamplified chromosome 17 polysomy in invasive breast carcinoma
·Gain-of-function PDGFRA mutations, earlier reported in gastrointestinal stromal tumors, are common in small intestinal inflammatory fibroid polyps. A study of 60 cases
·Fluorescence in situ hybridization as a tool for microstaging in malignant melanoma
·Papillary carcinoma of the breast lacks evidence of RET rearrangements despite morphological similarities to papillary thyroid carcinoma
·t(14;18)-negative follicular lymphomas are associated with a high frequency of BCL6 rearrangement at the alternative breakpoint region
·Lymphocyte-rich classical Hodgkin's lymphoma: distinctive tumor and microenvironment markers
·Adenomatoid tumors of the female and male genital tracts: a clinicopathological and immunohistochemical study of 44 cases
·Intercepting early pelvic serous carcinoma by routine pathological examination of the fimbria
·Differential protein immunoexpression profiles in appendiceal mucinous neoplasms: a special reference to classification and predictive factors
·Tumor-infiltrating lymphocytes in colorectal cancers with microsatellite instability are correlated with the number and spectrum of frameshift mutations
·Lymphoid neoplasms of the urinary tract and male genital organs: a clinicopathological study of 40 cases
·Distinction of hepatocellular adenoma from hepatocellular carcinoma with and without cirrhosis using E-cadherin and matrix metalloproteinase immunohistochemistry
·Most primary adrenal lymphomas are diffuse large B-cell lymphomas with non-germinal center B-cell phenotype, BCL6 gene rearrangement and poor prognosis
·Testicular mixed germ cell tumors: a morphological and immunohistochemical study using stem cell markers, OCT3/4, SOX2 and GDF3, with emphasis on morphologically difficult-to-classify areas
·HPV infection and immunochemical detection of cell-cycle markers in verrucous carcinoma of the penis
·Comparison of automated silver enhanced in situ hybridization and fluorescence in situ hybridization for evaluation of epidermal growth factor receptor status in human glioblastomas
·Cathepsin-K immunoreactivity distinguishes MiTF/TFE family renal translocation carcinomas from other renal carcinomas
·Clinical validation of an array CGH test for HER2 status in breast cancer reveals that polysomy 17 is a rare event
·Application of COLD-PCR for improved detection of KRAS mutations in clinical samples
·Fluorescence in situ hybridization study shows association of PTEN deletion with ERG rearrangement during prostate cancer progression
·Chromosomal copy number changes supporting the classification of lentiginous junctional melanoma of the elderly as a subtype of melanoma
·Characterization of ETS gene aberrations in select histologic variants of prostate carcinoma
·Nuclear expression of dynamin-related protein 1 in lung adenocarcinomas
·Prognostic impact of activated B-cell focused classification in diffuse large B-cell lymphoma patients treated with R-CHOP
·PAX2 expression in low malignant potential ovarian tumors and low-grade ovarian serous carcinomas
·Comparison of fluorescent in situ hybridization HER-2/neu results on core needle biopsy and excisional biopsy in primary breast cancer
·Analysis of the fibroblastic growth factor receptor-RAS/RAF/MEK/ERK-ETS2/brachyury signalling pathway in chordomas
·Candidate serous cancer precursors in fallopian tube epithelium of BRCA1/2 mutation carriers
·Expression of PAX8 in normal and neoplastic renal tissues: an immunohistochemical study

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Histopathology

·Molecular assessment of sentinel lymph node in breast cancer management
·Recent advances in molecular gynaecological pathology
·Gallbladder cancer: a morphological and molecular update
·Vascular invasion in breast cancer; an overview of recent prognostic developments and molecular pathophysiological mechanisms
·Prognostic significance of the therapeutic targets histone deacetylase 1, 2, 6 and acetylated histone H4 in cutaneous T-cell lymphoma

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Forums
Last 20 Forum Messages

Fluorescent doublestaining
Last post by hrn in General Immunohistochemistry on Jul 02, 2009 at 09:38:03

double staining?
Last post by hrn in General Immunohistochemistry on Jul 02, 2009 at 09:24:06

Quenching Peroxide
Last post by margarete in General Immunohistochemistry on Jul 01, 2009 at 14:02:07

hrn Alpha B Crystallin images
Last post by hrn in General Immunohistochemistry on Jun 30, 2009 at 08:38:41

Several questions about in situ hybridization
Last post by NONO in General In Situ Hybridization on Jun 29, 2009 at 18:42:57

Use of irrelevant tissues....
Last post by Carl in General Immunohistochemistry on Jun 28, 2009 at 16:46:40

Can Household Microwave woven used for pretreatment of IHC?
Last post by Carl in General Immunohistochemistry on Jun 23, 2009 at 21:18:33

NF-Kappa B IHC No nuclear staining
Last post by sagins in General Immunohistochemistry on Jun 21, 2009 at 08:00:05

CD4, CD19 TUNEL IHC in FFPET
Last post by Carl in General Immunohistochemistry on Jun 19, 2009 at 19:55:25

help,help...schwann and endothelial cell markers
Last post by Carl in General Immunohistochemistry on Jun 18, 2009 at 20:55:21

immunohistochemical staining for VEGF expression in hamsters
Last post by xylenefumes in General Immunohistochemistry on Jun 18, 2009 at 20:12:49

Signal Enhancer
Last post by Chodelas in General Immunohistochemistry on Jun 15, 2009 at 01:17:02

Azide in HIER buffers?
Last post by Carl in General Immunohistochemistry on Jun 13, 2009 at 21:03:47

Intestine/Swiss Roll
Last post by Carl in General Immunohistochemistry on Jun 09, 2009 at 15:38:53

black soudan and toluidin blue
Last post by Carl in General Immunohistochemistry on Jun 09, 2009 at 13:30:13

Invitrogen's Phalloidin-Alexa conjugates
Last post by Carl in General Immunohistochemistry on Jun 07, 2009 at 17:48:27

alpha synuclein
Last post by Carl in General Immunohistochemistry on May 28, 2009 at 21:58:26

about riboprobe hybrid problem....
Last post by Laoz in General In Situ Hybridization on May 21, 2009 at 15:56:36

Any one can help
Last post by NKM2010 in General In Situ Hybridization on May 18, 2009 at 16:59:20

FISH with citric buffer pretreatment
Last post by gula in General In Situ Hybridization on May 17, 2009 at 11:22:29


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Wednesday, December 17
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Tuesday, March 25
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Monday, December 03
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Top10 Links
· 1: Anderson Lab In Situ Hybridization Protocols
· 2: Immunohistochemical Staining Methods
· 3: Detection of mRNA by in situ hybridization
· 4: 35S-labeled probe
· 5: Detection and amplification of FISH signal
· 6: DIG-labeled probe
· 7: Chromosome In Situ Hybridization using biotin labeled probes
· 8: In Situ Hybridization
· 9: Chick, mouse, and Xenopus two colour whole mount ISH
· 10: Fluorescence In Situ Hybridization (FISH)
 
Molecular Cancer
·The novel RASSF6 and RASSF10 candidate tumour suppressor genes are frequently epigenetically inactivated in childhood leukaemias
·Altered regulation of metabolic pathways in human lung cancer discerned by 13C stable isotope-resolved metabolomics (SIRM)
·Co-activation of hedgehog and AKT pathways promote tumorigenesis in zebrafish
·Effects of cIAP-1, cIAP-2 and XIAP triple knockdown on prostate cancer cell susceptibility to apoptosis, cell survival and proliferation
·Equivalent benefit of mTORC1 blockade and combined PI3K-mTOR blockade in a mouse model of tuberous sclerosis
·Heme oxygenase-1 and its metabolites affect pancreatic tumor growth in vivo
·Expression levels of HMGA2 in adipocytic tumors correlate with morphologic and cytogenetic subgroups
·Identification of novel Notch target genes in T cell leukaemia
·Discovery of gene expression-based pharmacodynamic biomarker for a p53 context-specific anti-tumor drug Wee1 inhibitor
·Antineoplastic effect of iodine in mammary cancer: participation of 6-iodolactone (6-IL) and peroxisome proliferator-activated receptors (PPAR)

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Immunohistochemistry In Situ Hybridization Immunostaining
Immunohistochemistry Images Tissue staining Pathology
Tumor Markers IHC Reagents Tissue Micro Array
Immunofluorescence Staining Positive Control Slide Polyclonal Antibody
Monoclonal Antibody IHC Staining Protocol In Situ Hybridization Images
Immunohistochemistry Protocol Immunofluorescence Immunohistochemical Staining
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