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Immunohistochemistry is a technique for identifying cellular
or tissue constituents (antigens)
by means of antigen-antibody interactions, the site of antibody binding being
identified
either by direct labelling of the antibody, or by use of a secondary labelling
method.
In Situ Hybridization techniques allow the demonstration of specific nucleic
acid sequences (genes) in their cellular environment.
Above: Human Papillomavirus DNA demonstrated by In Situ
Hybridisation (pink)
in epithelial cells identified by indirect immunofluorescence using antibody
against cytokeratin (green)
A Global Search Engine for Antibodies
Search and find monoclonal, polyclonal, primary, secondary and conjugated antibodies from more than 500 antibody manufacturers & suppliers worldwide:
Immunoportal Site Search
Modern Immunohistochemistry
Modern Immunohistochemistry (Cambridge Illustrated Surgical Pathology)
Diagnostic pathology is an inherently flawed science due to the many possible interpretations of various tissues. However, the application of immunohistochemical stains as a diagnostic tool has been widely used since the 1990s as an extremely effective ancillary technique that removes much subjectivity from the practice. In fact, immunohistochemistry has supplanted simple morphologic evaluation as the definitive diagnostic method for a wide array of tumor types. This book offers a new and modern atlas-based resource for this science. Every anatomic region is covered in detail, and major diseases contain side-by-side examples of other ancillary staining techniques for comparison. The text is geared toward both the resident and practitioner of anatomic pathology and is supplemented with histograms, algorithms, and guides to the application and interpretation of uncommon antigens and immunostains. Not only is the book illustrated with more than 600 high-quality photomicrographs, but a companion CD-ROM of all images in downloadable format is included.
Posted by admin on Friday, June 19 @ 21:13:45 CEST (62 reads) (Read More... | Score: 0)
Immunohistochemistry - NeuroMabs
The mission of the NeuroMab facility is to provide a unique neuroscience-based approach to generating mouse monoclonal antibodies optimized for use in mammalian brain (NeuroMabs). NeuroMabs are generated from mice immunized with synthetic and recombinant immunogens corresponding to components of the neuronal proteome as predicted from genomic and other large-scale cloning efforts. Comprehensive biochemical and immunohistochemical analyses of human, primate and non-primate mammalian brain are incorporated into the initial NeuroMab screening procedure. This yields a subset of mAbs that are optimized for use in the brain for immunocytochemical-based imaging studies of protein localization in adult, developing and pathological brain samples, for biochemical analyses of subunit composition and post-translational modifications of native brain proteins, and for proteomic analyses of native brain protein networks.
The initial goal of the facility for the next 5 years is to generate a library of novel NeuroMabs against 250-500 neuronal proteins, initially focusing on membrane proteins (receptors/channels/transporters), synaptic proteins, other neuronal signaling molecules, and proteins with established links to disease states. These NeuroMabs will then be produced on a large scale and made available to the neuroscience research community on an inexpensive basis as tissue culture supernatant or purified immunoglobulin by Antibodies Inc.
Posted by admin on Monday, March 09 @ 08:00:00 CET (345 reads) (Read More... | Score: 0)
Video Protocols
The Journal of Visualized Experiments (JoVE)
The Journal of Visualized Experiments (JoVE) has announced that its online video protocols will be indexed in the popular US National Library of Medicine repositories MEDLINE and PubMed.
Founder and chief executive Moshe Pritsker views the MEDLINE–PubMed listing as a sign that the scientific community has accepted video-based publications. "It was a very important decision for us, and for scientific publishing," he says.
Since JoVE was founded in 2006 with support from an angel investor, the journal has published more than 200 videos, most produced by professional videographers. It aims to improve the reproducibility of scientific results by using videos to clarify subtle experimental details. The journal was itself an experiment in video publishing and remains the only video-based scientific journal.
Immunocytochemistry: Human Neural Stem Cells
Immunocytochemistry is a very powerful and fairly straightforward method for determining the presence, subcellular localization, and relative abundance of an antigen of interest, most commonly a protein, in cultured cells. This protocol presents an easy-to-follow series of steps that will enable researchers to conserve primary and secondary antibodies while getting high quality, reproducible qualitative and quantitative data out of their staining. There are two aspects of this protocol that help to conserve the volume of antibody necessary for staining. For one, the cells are grown on small, circular coverslips that are placed in wells of a tissue culture plate. After fixation, the cells on coverslips can be removed from the wells of the plate. For antibody staining, the coverslip with cells is inverted onto a small drop of antibody solution on parafilm and is covered with a second piece of parafilm to prevent drying. Using this method, only ~25 μl of antibody solution is needed for each coverslip (or sample) to be stained. This protocol describes immunostaining of human neural stem/precursor cells (hNSPCs), but can be used for many other cell types.
Posted by admin on Wednesday, March 04 @ 11:44:39 CET (865 reads) (Read More... | 13630 bytes more | Score: 5)
In Situ Hybridization
Locked nucleic acid (LNA™)
This is a presentation of Exiqons miRCURY LNA™ microRNA Detection Probes for In Situ Hybridization.
Locked nucleic acid (LNA™) nucleosides are a class of nucleic acid analogues in which the ribose ring is “locked” by a methylene bridge connecting the 2’-O atom and the 4’-C atom. LNA™ nucleosides contain the six common nucleobases (T, C, G, A, U and mC) that appear in DNA and RNA and are able to form base pairs according to standard Watson-Crick base pairing rules. However, by “locking” the molecule with the methylene bridge the LNA™ is constrained in the ideal conformation for Watson-Crick binding. When incorporated into a DNA oligonucleotide, LNA™ therefore makes the pairing with a complementary nucleotide strand more rapid and increases the stability of the resulting duplex.
LNA™ Oligonucleotides
An LNA™-enhanced oligonucleotide is the ideal choice whenever short or very similar sequences need to be analyzed. The high affinity of an LNA™ oligonucleotide to its complementary sequence results in higher specificity and sensitivity than when using traditional DNA or RNA sequences. In many cases, LNA™-enhanced oligonucleotides can distinguish between sequences differing by a single nucleotide, which can be critical for the success of many experiments.
Posted by admin on Friday, February 20 @ 08:55:25 CET (477 reads) (Read More... | 3252 bytes more | Score: 0)
Immunohistochemistry & In Situ Hybridization
Signal Amplifiers UltraAmp™ Multi-Assay Signal Amplifiers
Genisphere’s UltraAmp Multi-Assay Signal Amplifier opens up endless possibilities for high sensitivity detection. UltraAmp reagents are available with multiple different detection labels and targeting moieties, and come in different sizes to meet your specific assay design. The sensitivity improvement obtained with this single tube reagent is up to 200 fold, depending on the size of dendrimer, application, and nature of the assay. Genisphere has tested the UltraAmp products in several different assays (protein arrays, ELISA, Bead Flow (Luminex), and Nucleic Acid Arrays), to show the versatility of the detection reagent, but ultimately the application is up to you.
Genisphere’s new universal UltraAmp Amplification reagents drop right into your existing assay and improve your sensitivity up to 200 fold! These reagents come with different labels, target moieties and sizes to accommodate limitless assays’ needs for more sensitivity. There are over 70 different UltraAmp varieties to choose from.
A Global Search Engine for Antibodies
Search and find monoclonal, polyclonal, primary, secondary and conjugated antibodies from more than 500 antibody manufacturers & suppliers worldwide:
Diagnostic pathology is an inherently flawed science due to the many possible interpretations of various tissues. However, the application of immunohistochemical stains as a diagnostic tool has been widely used since the 1990s as an extremely effective ancillary technique that removes much subjectivity from the practice. In fact, immunohistochemistry has supplanted simple morphologic evaluation as the definitive diagnostic method for a wide array of tumor types. 
The mission of the NeuroMab facility is to provide a unique neuroscience-based approach to generating mouse monoclonal antibodies optimized for use in mammalian brain (NeuroMabs). NeuroMabs are generated from mice immunized with synthetic and recombinant immunogens corresponding to components of the neuronal proteome as predicted from genomic and other large-scale cloning efforts. Comprehensive biochemical and immunohistochemical analyses of human, primate and non-primate mammalian brain are incorporated into the initial NeuroMab screening procedure. This yields a subset of mAbs that are optimized for use in the brain for immunocytochemical-based imaging studies of protein localization in adult, developing and pathological brain samples, for biochemical analyses of subunit composition and post-translational modifications of native brain proteins, and for proteomic analyses of native brain protein networks. 
