Product Overview
Anti-Phosphorilated Histone H2AX
Quality Level
100
biological source
mouse
antibody form
affinity purified immunoglobulin
clone
JBW301, monoclonal
species reactivity
vertebrates
packaging
antibody small pack of 25 μg
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
NP_002096
General description
Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
Specificity
Recognizes Histone H2A.X phosphorylated at Ser139.
Immunogen
peptide (C-KATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X
Application
Anti-phospho-Histone H2A.X (Ser139), clone JBW301 is a well published Mouse Monoclonal Antibody validated in ChIP, ICC, IF, WB. This purified mAb is highly specific for phospho-Histone H2A.X (Ser139) also known as H2AXS139p.
Research Sub Category
Histones
Histones
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Additional Referenced Applications:
Immunohistochemistry Analysis: A representative lot detected Histone H2A.X (pSer139) in RNF168-WT and RNF 168-SA/SEKI mice lung tissue sections (Paraffin). (Xe, X., et al. (2015) Nat. Cell Biol. 20 (3); 320-331).
Chromatin Immunoprecipitation, see Meier, Andreas, et al. EMBO J., 26: 2707-18 (2007) in technical information tab.
Chromatin Immunoprecipitation, see Meier, Andreas, et al. EMBO J., 26: 2707-18 (2007) in technical information tab.
