A skin punch biopsy needle can be used to cut out representative tissue from preferable normal tissue blocks.
Composition, size and number may vary, but 2-6mm cores are normally sufficient.
Optimally, a 6mm core is replaced by several 2-4mm cores to cover weak, intermediate and strong expression of antigen.
Following Multi Tissue Control Blocks are suggestions from Nordic Immunohistochemical Quality Control NordiQC
|
APPENDIX |
TONSIL |
PANCREAS |
HEPAR |
|
|
AAT |
Macrophages & villi of columnar epithelial cells |
Macrophages |
0 |
Kupfer cells & hepatocytes (globuli) |
|
ASMA |
Smooth muscle cells in vessels and in muscle layers. Myofibroblasts lining the epithelial surface |
Smooth muscle cells in vessels |
Smooth muscle cells in vessels |
Smooth muscle cells in sinusoids |
|
B-CATENIN Beta-catenin (Membrane) |
Membranes of columnar epithelial cells. Endothelial and follicular denritic cells |
Membranes of squamous epithelial cells |
Membranes of acinar epithelial cells (ducts) and endocrine cells |
Hepatocytes - weak membranous |
|
BCL2 Bcl2-oncoprotein (Cytopl) |
All peripheral lymphocytes incl T-cells in germinal centres -Germinal centre B-cells are negative. |
All peripheral lymphocytes incl T-cells in germinal centres -Germinal centre B-cells are negative. |
Weak reaction of epithelial cells. |
0 |
|
BCL6 Bcl6-protein (Nuclear) |
Germinal centre B-cells |
Germinal centre B-cells and basal squamous cells |
0 |
0 |
|
BOB.1 BOB.1-protein (Nuclear) |
Strong reaction in germinal centre B-cells -weak reaction in mantle zone B-cells and plasma cells |
Strong reaction in germinal centre B-cells-weak reaction in mantle zone B-cells and plasma cells |
0 |
0 |
|
CD2 (Membrane) |
All T-cells -Isolated T-cell in germinal centres |
All T-cells -Isolated T-cells in germinal centres |
T-cells |
T-cells |
|
CD3 (Membrane) |
All T-cells -Isolated T-cell in germinal centres |
All T-cells -Isolated T-cell in germinal centres |
T-cells |
T-cells |
|
CD4 (Membrane) |
60 - 80 % of T-cells |
60 - 80 % of T-cells and Germinal centre macrophages |
0 |
Kupfercells and sinusoid endothelial cells |
|
CD5 (Membrane) |
All T-cells -Isolated T-cell in germinal centres |
All T-cells -Isolated T-cells in germinal centres |
T-cells |
T-cells |
|
CD7 (Membrane) |
Almost all T-cells |
Almost all T-cells |
T-cells |
T-cells |
|
CD8 (Membrane & cytopl) |
20 - 40 % of the T-cells and NK-cells, Isolated T-cells in germinal centres |
20 - 40 % of the T-cells and NK-cells, Isolated T-cells in germinal centres |
0 |
0 |
|
CD10 (Membrane) |
Germinal centre B-cells, neutrophile granulocytes |
Germinal centre B-cells, neutrophile granulocytes |
0 |
Gall canaliculi |
|
CD14 (Membrane) |
Follicular dendritic cells and macrophages |
Follicular dendritic cells and macrophages |
Macrophages |
Kupfercells and sinusoid endothelial cells |
|
CD20 (Membrane) |
All B-cells incl germinal centre B-cells, plasma cells are neg. |
All B-cells incl germinal centre B-cells, plasma cells are neg. |
B-cells |
B-cells |
|
CD21 (Membrane) |
Follicular dendritic cells |
Follicular dendritic cells and a few mantle zone B-cells |
0 |
0 |
|
CD23 (Membrane) |
Follicular dendritic cells and 10 - 50 % of the mantle zone B-cells |
Follicular dendritic cells and 10 - 50 % of the mantle zone B-cells |
0 |
0 |
|
CD31 (Membrane & cytopl) |
Endothelial cells and a few mantle zone B-cells |
Endothelial cells and a few mantle zone B-cells |
Endothelial cells |
Endothelial cells |
|
CD34 (Membrane & cytopl) |
Endothelial cells and myofibroblasts |
Endothelial cells and myofibroblasts |
Endothelial cells and myofibroblasts |
Portal endothelial cells |
|
CD38 (Membrane) |
Germinal centre B-cells and plasma cells |
Germinal centre B-cells and plasma cells |
Plasma cells |
Plasma cells and weak reaction of sinusoids |
|
CD43 (Membrane) |
Almost all T-cells |
Almost all T-cells -Isolated T-cells in germinal centres |
T-cells |
T-cells |
|
CD45 Leucocyt common antigen (Membrane) |
All lymphocytes |
All lymphocytes |
All lymphocytes |
Lymphocytes and Kupfer cells |
|
CD56 (Membrane) |
Peripheral nerves -ganglion cells and axons. Endocrine cells |
NK-cells in the interfollicular areas |
Peripheral nerves and the islets of Langerhans |
Peripheral nerves |
|
CD57 (Membrane) |
Peripheral nerves -ganglion cells and axons. Endocrine cells |
NK-cells in the interfollicular areas and T-cells in germinal centres |
Peripheral nerves and a few cells in the islets of Langerhans |
0 |
|
CD68 (Membrane & cytopl) |
Granulocytes and germinal centre macrophages |
Granulocytes and germinal centre macrophages |
Granulocytes |
Kupfer cells |
|
CD79 (Membrane & cytopl) |
All B-cells incl germinal centre cells and plasma cells |
All B-cells incl germinal centre cells and plasma cells |
All B-cells |
All B-cells |
|
CD117 (Membrane & cytopl) |
Mast cells and Cajal cells- smooth muscle cells shall be negative |
Mast cells |
Mast cells |
Mast cells |
|
CD138 (Membrane & cytopl) |
Plasma cellsand a weak reaction of the columnar epithelial cells |
Plasma cells |
Plasma cells |
Plasma cells |
|
Cdx2 (Nuclear) |
All nuclei of columnar epithelial cells |
Negative |
Weak reaction of epithelial cells in large ducts |
Negative - occasionally few gall epithelial cells are pos. |
|
CEA Carcino-embryonic antigen, mono (Cytopl) |
All columnar epithelial cells - luminal accentuation and reaction of the cytoplasm |
Squamous epithelial cells |
0 |
0 |
|
CEA PO Carcino-embryonic antigen, poly (Cytopl) |
All columnar epithelial cells - luminal accentuation and reaction of the cytoplasm |
Squamous epithelial cells, granulocytes and macrophages |
Granulocytes and macrophages |
Gall canaliculi and kupfer cells |
|
CGA-PO Chromogranin A, poly (Cytopl) |
Endocrine cells and peripheral nerves -ganglion cells and axons |
0 |
Endocrine cells in the islets of Langerhans |
Peripheral nerves. |
|
CGA Chromogranin A, mono (Cytopl) |
Endocrine cells and peripheral nerves -ganglion cells and axons |
0 |
Endocrine cells in the islets of Langerhans |
Peripheral nerves. |
|
CK5 cytokeratin 5 (Cytopl) |
Mesothelial cells |
Squamous epithelial cells. |
Negative |
Negative |
|
CK5/6 Cytokeratin 5/6 (Cytopl) |
Mesothelial cells |
Squamous epithelial cells. |
Negative |
Negative |
|
CK7 Cytokeratin 7 (Cytopl) |
Mesothelial cells -occasionally a few columnar epithelial and endothelial cells. |
Squamous epithelial cells |
Columnar epithelial cells of large ducts and intercalated ducts |
Gall columnar epithelial cells |
|
CK8 Cytokeratin 8 (Cytopl) |
Columnar epithelial cells & mesothelial cells |
A few squamous epithelial cells and focally fibroblastic reticulum cells |
All columnar epithelial cells and islets of Langerhans |
Gall columnar epithelial cells & hepatocytes |
|
CK13 Cytokeratin 13 (Cytopl) |
Negative |
Suprabasal squamous epithelial cells. |
Negative |
Negative |
|
CK14 Cytokeratin 14 (Cytopl) |
Mesothelial cells |
Squamous epithelial cells. |
Negative |
Negative |
|
CK18 Cytokeratin 18 (Cytopl) |
Columnar epithelial cells & mesothelial cells |
Few squamous epithelial cells and focally fibroblastic reticulum cells |
All columnar epithelial cells and islets of Langerhans |
Gall columnar epithelial cells & hepatocytes |
|
CK19 Cytokeratin 19 (Cytopl) |
Columnar epithelial cells & mesothelial cells |
Few squamous epithelial cells |
Columnar epithelial cells of large ducts |
Gall columnar epithelial cells |
|
CK20 Cytokeratin 20 (Cytopl) |
Columnar epithelial cells and endocrine cells |
0 |
0 |
0 |
|
CK Sentinel node mamma Cytokeratin 7 + 19 (Cytopl) |
Columnar epithelial cells & mesothelial cells. Occasionally endothelial cells. |
Few squamous epithelial cells |
Columnar epithelial cells of large ducts and intercalated ducts |
Gall columnar epithelial cells |
|
CK LMW (Cam5.2) Low mol weight cytokeratin (Cytopl) |
Columnar epithelial cells & mesothelial cells |
A few squamous epithelial cells and focally fibroblastic reticulum cells |
All columnar epithelial cells and islets of Langerhans |
Gall columnar epithelial cells & hepatocytes |
|
CK HMW (34BE12) High mol weight cytokeratin (Cytopl) |
Negative -A few columnar epithelial cells might be pos.Mesothelial cells are pos. |
Squamous epithelial cells |
Negative - A few columnar epithelial cells (ducts) might be pos |
Negative -gall epithelial cells can be pos. |
|
CK Pan (AE1/AE3) Pan cytokeratin (Cytopl) |
Columnar epithelial cells & mesothelial cells |
Squamous epithelial cells and focally fibroblastic reticulum cells) |
All columnar epithelial cells and islets of Langerhans |
Gall columnar epithelial cells & hepatocytes |
|
CK Pan (KL1) Pan cytokeratin (Cytopl) |
Columnar epithelial cells & mesothelial cells |
Squamous epithelial cells and focally fibroblastic reticulum cells) |
All columnar epithelial cells and islets of Langerhans |
Gall columnar epithelial cells & hepatocytes |
|
CK Pan (MNF116) Pan cytokeratin (Cytopl) |
Columnar epithelial cells & mesothelial cells |
Squamous epithelial cells and focally fibroblastic reticulum cells) |
All columnar epithelial cells and islets of Langerhans |
Gall columnar epithelial cells & hepatocytes |
|
COL IV Collagen IV (Extra cel) |
Basal membrane under the epithelial surface and muscle cells |
Basal membrane under the epithelial surface |
Muscle cells |
Muscle cells |
|
CR Calretinin (Cytopl & nuclear) |
Peripheral nerves -ganglion cells and axons - ,macrophages and mesothelial cells. |
Macrophages |
Peripheral nerves -ganglion cells and axons -, macrophages |
0 |
|
CYD1 Cyclin D1 (Nuclear) |
Few basal columnar epithelial cells |
Parabasal squamous epithelial cells andfew fibroblastic cells |
Few basal columnar epithelial cells |
0 |