Product Overview
CRISPR/Cas9 Polyclonal Antibody
Cas9 Antibodies
Antibodies that detect Cas9 can be used in several scientific applications, including Western Blot, Immunocytochemistry, ELISA, Immunoprecipitation and Flow Cytometry. Our Cas9 monoclonal, polyclonal, recombinant monoclonal and recombinant polyclonal antibodies are developed in Mouse and Rabbit.
These antibodies have been verified by Relative expression to confirm specificity to Cas9. Find the Cas9 antibody that fits your needs. Choose from 1 of 40 Cas9 antibodies, which have been validated in experiments with 14 publications and 104 images featured in our data gallery.
Browse primary antibodies for WB, Flow, IHC, ICC/IF, ELISA, IP, and other applications. Antibodies with Advanced Verification data have been validated for specificity to ensure that the antibody binds to the antigen stated. If you cannot find the antibody you're looking for, contact us today to develop custom antibodies for specific targets, species and applications.
Target Information
Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering. The system is unique and flexible due to its dependence on RNA as the element that targets the nuclease to a desired DNA sequence.
It can be used to induce indel mutations, specific sequence replacements/insertions, and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation.
Cas9 may be derived from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9). SaCas9 has a number of properties that make it advantageous for genome editing, including its small size, high efficiency, nickase activity, and apparent specificity.
SaCas9 principally recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at rates comparable to SpCas9 with an ideal spacer lengths of 24 to 20 nucleotides.
In comparison to SpCas9, SaCas9 is smaller (1368 AA versus 1053 AA) and generates indels at a comparable rate when directed against target DNA with the mutually permissive NGGRRT PAM. This makes it the preferred enzyme for all-in-one delivery of Cas9 and multiple gRNA expression cassettes with Adeno-associated vectors.
Synonyms
cas9; CASP 9; Caspase9; Caspase-9 precursor; CASPASE-9c; CRISPR9; CRISPR-Cas9; CTD; OTTHUMP00000002324; RNCASP9; RP11-265F14.3; s aureus
