Product Overview
(Staphylococcus) Chicken Anti Protein A
biological source
rabbit
Quality Level
200
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
chicken
technique(s)
direct ELISA: 1:30,000
dot blot: 1:160,000 (indirect chemiluminescence)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000
shipped in
dry ice
Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus anti-chicken antibodies are useful analytical tools for various research applications.
Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody binds to chicken IgG.
Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody binds to chicken IgG.
Immunogen
purified chicken IgG
Application
Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody has been used for immunoprecipitation and western blot (1:2,000) assays. The antibody can also be used for direct ELISA (1:30,000), dot blot (1:12,000-1:15,000) and IHC (1:1,000) applications.
Proteins isolated from rabbit glomeruli by iron oxide magnetization were subjected to western blot analysis using HRP-conjugated rabbit anti-chicken IgY as the secondary antibody.
Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody produced in rabbit has been used in indirect enzyme-linked immunosorbent assay (ELISA), dierct ELISA, dot blot and immunohistochemistry.
Biochem/physiol Actions
Egg yolk IgY immunoglobulin acquired from hen have been studied extensively. It is useful in immunological assays and does interact with the rheumatoid factor.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT
Preparation Note
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
